The effect of ascorbic acid treatment on some biochemical properties of tobacco plants (Basma) under salt stress conditions
الكلمات المفتاحية:
Tobacco seeds, Basma, ascorbic, salt stress
الملخص
Soil salinity is a major factor that leads to low productivity of cultivated crops. Salinity causes oxidative stress
In plants by enhancing the production of reactive oxygen species. Ascorbic acid is an effective antioxidant that is essential for increasing the salt tolerance of plants. However, plant antioxidant responses to salinity vary widely in tobacco plants.
The current study was conducted to determine the effect of ascorbic acid on the tobacco plant (Basma). Which grows under salt water (12, 16 and 18 mm/cm2). Different concentrations of ascorbic acid (150, 300 and 450 mg/L) were applied.
Salt toxicity significantly reduced the growth of tobacco plants. The significant decrease in chlorophyll and carotenoids due to salinity was mitigated by the use of ascorbic acid. Which contributed to improving the plant’s content of proline, hydrogen peroxide, and soluble sugars. Among the concentrations, 150 ml/L mm was most effective in reducing salinity stress. Therefore, we can recommend spraying with ascorbic acid which improved the growth of tobacco plants under saline conditions.
References
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Azooz MM, Shaddad MA, Abdel Latef AA. The accumulation and compartmentation of proline in relation to salt tolerance of three sorghum cultivars. Indian J Plant Physi 9: 1-8, 2004.
Balsamo, R.A.; Thomson, W.W. (1995). Salt effect on membrane of the hypodermis and mesophyll cells of Avicennia germinans (Avicenniaceae): a freeze-fracture study. American Journal of Botany 4: 435–440.
Bandehagh A, Toorchi M, Mohammadi A et al. Growth and osmotic adjustment of canola genotypes in response to salinity. J Food Agric Environ 6: 201-208, 2008.
Bates, L.S.; R.P. Waldren and I.D. Tear (1973). Rapid determination of free proline for water-stress studies. Plant and Soil. 39: 205–207.
Camlica, M. and Yaldiz, G. (2021). Analyses and evaluation of the main chemical components in different tobacco (Nicotiana tabacum L.) genotypes. Grasas y Aceites, 72(1), e389-e389.
Celik O, Atak C. The effect of salt stress on antioxidative enzymes and proline content of two Turkish tobacco verities. Turk J Biol 36: 327-338, 2012.
Ĉerný, M., Habánová, H., Berka, M., Luklová, M. and Brzobohatý, B. (2018).
Hydrogen peroxide: Its role in plant biology and crosstalk with signaling
networks. Int. J. Mol. Sci. 19, 2812.
Dubois, M.; Gilles, K.A.; Hamelton, J.K.; Rebers, P.A.; Smith, F. (1956) . Chlorometric method for determination of sugars and related substrates . Anal. Chem., 28 : 350 – 356.
Gurmani AR, Bano A, Salim M. Effect of growth regulators on growth, yield and ion accumulation of rice (Oryza sativa L.) under salt stress. Pak J Bot 38: 1415-1424, 2006.
Hamada, A. M. 1998. Effect of exogenously added ascorbic acid, thiamin or asprin on photosynthesis and some related activities of drought-stressed wheat plants. In: Photosynthesis: Mechanisms and Effects. Garab, G. (ed.). Kluwer Academic Publishers, Dordrecht. p. 2581-2584.
Hamada, A.M.; Al-Hakimi, A.M.A. (2001). Salicylic acid versus salinity-drought induced stress on wheat seedlings. Rostlinna Vyroba, 47: 444-450.
Hussein MM, Balbaa LK, Gaballah MS. Salicylic acid and salinity effects on growth of maize plants. Res J Agric Biol Sci 3: 321-328, 2007.
Koca H, Bor M, Özdemir F et al. Th e eff ect of salt stress on lipid peroxidation, antioxidative enzymes, and proline content of sesame cultivars. Environ Exp Bot 60: 344-351, 2007.
Lichtenthaler, H.K. (1987). Chlorophylls and carotenoids pigments of photosynthesis biomebranes. In: Colowick, S.P.; Kaplan, N.O. (eds.). Methods in Enzymology. Academic Press, New York, pp 350–38.
Liu, L., Li, D., Ma, Y., Shen, H., Zhao, S. and Wang, Y. (2021). Combined application of arbuscular mycorrhizal fungi and exogenous melatonin alleviates drought stress and improves plant growth in tobacco seedlings. Journal of Plant Growth Regulation, 40(3), 1074-1087.
Misra, A.N.; Sahu, S. M.; Mishra, M.; Singh, P.; Meera, I.; Das, N.; Kar, M.; Sahu, P. (1997). Sodium chloride induced changes in leaf growth and pigment and protein contents in two rice cultivars. Biologia Plantarum 39: 257–262.
Molinari HBC, Marur CJ, Daros E et al. Evaluation of the stress-inducible production of proline in transgenic sugarcane (Saccharum spp.): osmotic adjustment, chlorophyll fluorescence and oxidative stress. Physiol Plantarum 130: 218- 229, 2007.
Nanda, C., Sarala, K., Nagesh, P. and Ramakrishnan, S. (2021). Heritability and genetic variability studies in the germplasm accessions of flue cured Virginia tobacco (Nicotiana tobaccum L.). Emergent Life Sci. Res. 7, 36–39.
Noctor, G. and C. H. Foyer. 1998. Ascorbate and glutathione: Keeping active oxygen under control. Annu. Rev. Pl. Physiol. Pl. Mol. Biol. 49: 249-279.
Pignocchi, C., Kiddle, G., Hernández, I., Foster, S. J., Asensi, A. and Taybi, T. (2006) Ascorbate oxidase-dependent changes in the redox state ofthe apoplast modulate gene transcript accumulation leading to modified hormone signaling and orchestration of defense processes in tobacco. Plant Physiol. 141: 423–435
Regassa, R. and Chandravanshi, B.S. (2016). Levels of heavy metals in the raw and processed Ethiopian tobacco leaves. Springer Plus 5, 232.
Sairam, R. K., K.V. Rao and G.C. Srivastava. 2002. Differential response of wheat genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration. Pl. Sci. 163: 1037-1046.
Sekmen, A. H., I. Tu¨rkan and S. Takio. 2007. Differential responses of antioxidative enzymes and lipid peroxidation to salt stress in salttolerant Plantago maritima L. and salt-sensitive Plantago media L. Physiol. Pl. 131:399-411.
Shaddad, M.A., A.F. Radi, A.M. Abdel-Rahman and M.M. Azooz (1999) Response of seeds of lupines termas and Vicia faba to the interactive effect of salinity and ascorbic acid or pyridoxine. Plant & Soil, 122 (2): 177-183.
Skopelitis DS, Paranychianakis NV, Paschalidis KA et al. Abiotic stress generates ROS that signal expression of anionic glutamate dehydrogenases to form glutamate for proline synthesis in tobacco and grapevine. Th e Plant Cell 18: 2767- 2781, 2006.
Tang, Z., Chen, L., Chen, Z., Fu, Y., Sun, X., Wang, B. and Xia, T. (2020). Climatic factors determine the yield and quality of Honghe flue-cured tobacco. Sci. Rep., 10, 19868.
Turan MA, Elkarim AHA, Taban N et al. Effect of salt stress on growth, stomatal resistance, proline and chlorophyll concentrations on maize plant. Afr J Agric Res 4: 893-897, 2009.
Turner, J.E.; Begg, J.E. (1978). Responses of pasture plants to water deficits. In: Wilson J.R. (ed.): Plants Relations in Pastures. CSIRO, Melbourne, pp. 50-66.
Velikova, V.; I. Yordanov and A. Edreva (2000). Oxidative stress and some antioxidant systems in acid rain-treated bean plants: Protective role of exogenous polyamines. Plant Science. 151: 59–66.
Verma, S.; Dubey, R.S. (2003). Lead toxicity induces lipid peroxidation and alters the activities of antioxidant enzymes in growing rice plants. Plant Science 164: 645–655.
Wang, J., Zhang, Z., and Huang, R. (2013) Regulation of ascorbic acid synthesis in plants. Plant Signal. Behav. 8:e24536. doi: 10.4161/psb.24536.
Ye, N., Zhu, G., Liu, Y., Zhang, A., Li, Y., Liu, R., (2012) Ascorbic acid and reactive oxygen species are involved in the inhibition of seed germination by abscisic acid in rice seeds. J. Exp. Bot. 63: 1809– 1822.
Azooz MM, Shaddad MA, Abdel Latef AA. The accumulation and compartmentation of proline in relation to salt tolerance of three sorghum cultivars. Indian J Plant Physi 9: 1-8, 2004.
Balsamo, R.A.; Thomson, W.W. (1995). Salt effect on membrane of the hypodermis and mesophyll cells of Avicennia germinans (Avicenniaceae): a freeze-fracture study. American Journal of Botany 4: 435–440.
Bandehagh A, Toorchi M, Mohammadi A et al. Growth and osmotic adjustment of canola genotypes in response to salinity. J Food Agric Environ 6: 201-208, 2008.
Bates, L.S.; R.P. Waldren and I.D. Tear (1973). Rapid determination of free proline for water-stress studies. Plant and Soil. 39: 205–207.
Camlica, M. and Yaldiz, G. (2021). Analyses and evaluation of the main chemical components in different tobacco (Nicotiana tabacum L.) genotypes. Grasas y Aceites, 72(1), e389-e389.
Celik O, Atak C. The effect of salt stress on antioxidative enzymes and proline content of two Turkish tobacco verities. Turk J Biol 36: 327-338, 2012.
Ĉerný, M., Habánová, H., Berka, M., Luklová, M. and Brzobohatý, B. (2018).
Hydrogen peroxide: Its role in plant biology and crosstalk with signaling
networks. Int. J. Mol. Sci. 19, 2812.
Dubois, M.; Gilles, K.A.; Hamelton, J.K.; Rebers, P.A.; Smith, F. (1956) . Chlorometric method for determination of sugars and related substrates . Anal. Chem., 28 : 350 – 356.
Gurmani AR, Bano A, Salim M. Effect of growth regulators on growth, yield and ion accumulation of rice (Oryza sativa L.) under salt stress. Pak J Bot 38: 1415-1424, 2006.
Hamada, A. M. 1998. Effect of exogenously added ascorbic acid, thiamin or asprin on photosynthesis and some related activities of drought-stressed wheat plants. In: Photosynthesis: Mechanisms and Effects. Garab, G. (ed.). Kluwer Academic Publishers, Dordrecht. p. 2581-2584.
Hamada, A.M.; Al-Hakimi, A.M.A. (2001). Salicylic acid versus salinity-drought induced stress on wheat seedlings. Rostlinna Vyroba, 47: 444-450.
Hussein MM, Balbaa LK, Gaballah MS. Salicylic acid and salinity effects on growth of maize plants. Res J Agric Biol Sci 3: 321-328, 2007.
Koca H, Bor M, Özdemir F et al. Th e eff ect of salt stress on lipid peroxidation, antioxidative enzymes, and proline content of sesame cultivars. Environ Exp Bot 60: 344-351, 2007.
Lichtenthaler, H.K. (1987). Chlorophylls and carotenoids pigments of photosynthesis biomebranes. In: Colowick, S.P.; Kaplan, N.O. (eds.). Methods in Enzymology. Academic Press, New York, pp 350–38.
Liu, L., Li, D., Ma, Y., Shen, H., Zhao, S. and Wang, Y. (2021). Combined application of arbuscular mycorrhizal fungi and exogenous melatonin alleviates drought stress and improves plant growth in tobacco seedlings. Journal of Plant Growth Regulation, 40(3), 1074-1087.
Misra, A.N.; Sahu, S. M.; Mishra, M.; Singh, P.; Meera, I.; Das, N.; Kar, M.; Sahu, P. (1997). Sodium chloride induced changes in leaf growth and pigment and protein contents in two rice cultivars. Biologia Plantarum 39: 257–262.
Molinari HBC, Marur CJ, Daros E et al. Evaluation of the stress-inducible production of proline in transgenic sugarcane (Saccharum spp.): osmotic adjustment, chlorophyll fluorescence and oxidative stress. Physiol Plantarum 130: 218- 229, 2007.
Nanda, C., Sarala, K., Nagesh, P. and Ramakrishnan, S. (2021). Heritability and genetic variability studies in the germplasm accessions of flue cured Virginia tobacco (Nicotiana tobaccum L.). Emergent Life Sci. Res. 7, 36–39.
Noctor, G. and C. H. Foyer. 1998. Ascorbate and glutathione: Keeping active oxygen under control. Annu. Rev. Pl. Physiol. Pl. Mol. Biol. 49: 249-279.
Pignocchi, C., Kiddle, G., Hernández, I., Foster, S. J., Asensi, A. and Taybi, T. (2006) Ascorbate oxidase-dependent changes in the redox state ofthe apoplast modulate gene transcript accumulation leading to modified hormone signaling and orchestration of defense processes in tobacco. Plant Physiol. 141: 423–435
Regassa, R. and Chandravanshi, B.S. (2016). Levels of heavy metals in the raw and processed Ethiopian tobacco leaves. Springer Plus 5, 232.
Sairam, R. K., K.V. Rao and G.C. Srivastava. 2002. Differential response of wheat genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration. Pl. Sci. 163: 1037-1046.
Sekmen, A. H., I. Tu¨rkan and S. Takio. 2007. Differential responses of antioxidative enzymes and lipid peroxidation to salt stress in salttolerant Plantago maritima L. and salt-sensitive Plantago media L. Physiol. Pl. 131:399-411.
Shaddad, M.A., A.F. Radi, A.M. Abdel-Rahman and M.M. Azooz (1999) Response of seeds of lupines termas and Vicia faba to the interactive effect of salinity and ascorbic acid or pyridoxine. Plant & Soil, 122 (2): 177-183.
Skopelitis DS, Paranychianakis NV, Paschalidis KA et al. Abiotic stress generates ROS that signal expression of anionic glutamate dehydrogenases to form glutamate for proline synthesis in tobacco and grapevine. Th e Plant Cell 18: 2767- 2781, 2006.
Tang, Z., Chen, L., Chen, Z., Fu, Y., Sun, X., Wang, B. and Xia, T. (2020). Climatic factors determine the yield and quality of Honghe flue-cured tobacco. Sci. Rep., 10, 19868.
Turan MA, Elkarim AHA, Taban N et al. Effect of salt stress on growth, stomatal resistance, proline and chlorophyll concentrations on maize plant. Afr J Agric Res 4: 893-897, 2009.
Turner, J.E.; Begg, J.E. (1978). Responses of pasture plants to water deficits. In: Wilson J.R. (ed.): Plants Relations in Pastures. CSIRO, Melbourne, pp. 50-66.
Velikova, V.; I. Yordanov and A. Edreva (2000). Oxidative stress and some antioxidant systems in acid rain-treated bean plants: Protective role of exogenous polyamines. Plant Science. 151: 59–66.
Verma, S.; Dubey, R.S. (2003). Lead toxicity induces lipid peroxidation and alters the activities of antioxidant enzymes in growing rice plants. Plant Science 164: 645–655.
Wang, J., Zhang, Z., and Huang, R. (2013) Regulation of ascorbic acid synthesis in plants. Plant Signal. Behav. 8:e24536. doi: 10.4161/psb.24536.
Ye, N., Zhu, G., Liu, Y., Zhang, A., Li, Y., Liu, R., (2012) Ascorbic acid and reactive oxygen species are involved in the inhibition of seed germination by abscisic acid in rice seeds. J. Exp. Bot. 63: 1809– 1822.
منشور
2025-07-15
How to Cite
صوفانق. (2025). The effect of ascorbic acid treatment on some biochemical properties of tobacco plants (Basma) under salt stress conditions. Journal of Hama University , 7(21). Retrieved from https://hama-univ.edu.sy/ojs/index.php/huj/article/view/2144
القسم
Department of Agriculture Engineering
