The Effect of Using L-Arginine as a Feed Additive on Testosterone Levels in Ross 308 Broiler Breeder Roosters
The Effect of Using Arginine on Testosterone Level in Roosters of Broiler Breeders
الكلمات المفتاحية:
Key words: arginine- dietary supplement- functional performance- reproductive system- broiler breeder roosters- testosterone- sperm motility
الملخص
This study was conducted to demonstrate the effect of using arginine as a dietary supplement on the functional performance of the reproductive system in broiler breeder roosters. For this study, 60 broiler breeder roosters of the Ross 308 strain were used, and the birds were divided into four groups. The control group included 15 birds fed according to the recommendations of the strain's production company. Group S1 included 15 birds fed according to the company's recommendations with the addition of arginine at a rate of 1 kg per ton of feed. The second group, S2, also included 15 birds, with arginine added to the feed mixture at a rate of 2 kg per ton of feed. The fourth group, S3, included 15 birds, with arginine added at a rate of 4 kg per ton of feed.
Blood samples were taken to examine testosterone levels at four different time points: at the beginning of the experiment, then after 15, 30, and 60 days from the start of the experiment. The results showed an increase in testosterone levels, sperm motility, and an improvement in seminiferous tubule diameter in groups S2 and S3 compared to the control group and S1.
References
المراجع:
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21. Lundberg, J. O., Weitzberg, E., & Gladwin, M. T. (2008). The nitrate-nitrite-nitric oxide pathway. Nature Reviews Drug Discovery, 7(2), 156–167.
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23. Patel, A. B., Srivastava, S., Phadke, R. S., & Govil, G. (1998). Arginine activates glycolysis of goat epididymal spermatozoa: An NMR study. Biophysical Journal, (3) 75, 1522–1528.
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27. Silva, L. M. G. S., Murakami, A. E., Fernandez, J. I. M., Dalla Rossa, D., & Urgnani, F. J. (2012). Effects of dietary arginine supplementation on broiler breeder egg production and hatchability. Brazilian Journal of Poultry Science,14(4), 233–304.
28. Soares, R. D. A. T., Fonseca, J. B., Santos, A. S. O., & Mercandante, M. B. (2003). Protein requirement of Japanese quail (Coturnix coturnix japonica) during rearing and laying periods. Brazilian Journal of Poultry Science, 5 153–156.
29. Tamir, H., Ratner, S., & Bengal, E. (2005). Metabolic constraints on arginine biosynthesis in avian species. Journal of Nutrition, 135(5), 1238–1245.
30. Traish, A. M., Toselli, P., Jeong, S. J., & Kim, N. N. (2018). Arginine supplementation in male infertility. International Journal of Impotence Research, 30(1), 1–8.
31. Wu, G., & Morris, S. M. (1998). Arginine metabolism: Nitric oxide and beyond. Annual Review of Nutrition, 18, 61–86.
32. Wu, G., Bazer, F. W., Davis, T. A., Kim, S. W., Li, P., Marc Rhoads, J., Carey Satterfield, M., Smith, S. B., Spencer, T. E., & Yin, Y. (2023). Individual variability in response to arginine. Nutrients, 15(2), 325.
33. Yang, H., Ju, X., Wang, Z., Yang, Z., Lu, J., & Wang, W. (2016). Effects of arginine supplementation on organ development, egg quality, serum biochemical parameters, and immune status of laying hens. Revista Brasileira de Ciência Avícola, 18(1), 181–186.
34. Yang, B., Li, X., Mesalam, N. M., Elsadek, M. F., & Abdel-Moneim, A. M. E. (2024). The impact of dietary supplementation of polysaccharide derived from Polygonatum sibiricum on growth, antioxidant capacity, meat quality, digestive physiology, and gut microbiota in broiler chickens. Poultry Science, 103(6), 103675.
35. Zhou, Q., Li, M., Wang, X., Li, Q., Wang, T., Zhu, Q., Zhou, X., Wang, Y., Gao, T., & Wang, J. (2019). Arginine and nitric oxide synthase in poultry. Poultry Science, 98(11), 5475–5481..
1. Adman, M. (1970). Effect of arginine on oligospermia. Fertility and Sterility, 21, 217–219.
2. Aitken, R. J., Smith, T. B., Jobling, M. S., Baker, M. A., & De Iuliis, G. N. (2015). Oxidative stress and male reproductive health. Asian Journal of Andrology, 17(2), 31–38.
3. Alderton, W. K., Cooper, C. E., & Knowles, R. G. (2001). Nitric oxide synthases: Structure, function and inhibition. Biochemical Journal, 357(3), 593–615.
4. Aydin, M., Keles, I., Yilmaz, B., Odaci, E., & Kose, E. (2016). L-arginine and antioxidant supplementation on testicular function. Journal of Animal Physiology and Animal Nutrition, 100(1), 162–170.
5. Barb, C. R., Kraeling, R. R., & Rampacek, G. B. (1995). Role of neuropeptides in control of growth hormone secretion in swine. Livestock Production Science, 42(2-3), 129–136.
6. Brown, K. M., & Arthur, J. R. (2001). Selenium, selenoproteins and human health: A review. *Public Health Nutrition, 4(2b), 593–599.
7. Burnett, A. L., Lowenstein, C. J., Bredt, D. S., Chang, T. S., & Snyder, S. H. (2017). Nitric oxide in penile erection. Nature Reviews Urology, 14(10), 643–653.
8. Chen, J., Zhang, Y., Wang, C., Lü, W., & Jin, Y. (2020). Arginine-NO pathway and testosterone synthesis. Poultry Science, 99(9), 4387–4394.
9. Damerow, G. (2015). The chicken encyclopedia: An illustrated reference, Storey Publishing.
10. El-Boshy, M., El-Sayed, R., El-Gazzar, A., & El-Sayed, A. (2022). Antioxidant effects of arginine in testis. BMC Veterinary Research, 18(1), 123.
11. Fischmann, T. O., Hruza, A., Niu, X. D., Fossetta, J. D., Lunn, C. A., Dolphin, E., Prongay, A. J., Reichert, P., Lundell, D. J., Narula, S. K., & Weber, P. C. (1999). Structural characterization of nitric oxide synthase isoforms reveals striking active-site conservation. *Nature Structural Biology, 6(3), 233–242.
12. Fletcher, M., Ramirez, M. E., Sierra, R., Raber, P., & Rodriguez, P. (2013). Modulation of T cell function through L-arginine metabolism: A new therapy from an old enemy. Journal for Immunotherapy of Cancer, 1(1), 10.
13. Förstermann, U., & Sessa, W. C. (2012). Nitric oxide synthases: Regulation and function. European Heart Journal,33(7), 829–837.
14. Garcin, E. D., Bruns, C. M., Lloyd, S. J., Hosfield, D. J., Tiso, M., Gachhui, R., Stuehr, D. J., Tainer, J. A., & Getzoff, E. D. (2004). Structural basis for isozyme-specific regulation of electron transfer in nitric-oxide synthase. Journal of Biological Chemistry, 279(36), 37918–37927.
15. Gonzalez-Cadavid, N. F., & Rajfer, J. (2004). Molecular pathophysiology of erectile dysfunction. Current Opinion in Urology, 14(6), 419–427.
16. Ibrahim, H. A., Zhu, Y., Wu, C., Lu, C., Ezekwe, M. O., Liao, S. F., & Huang, K. (2020). Dose-dependent effects of arginine on male reproduction. Asian Journal of Andrology, 22(4), 350–357.
17. Ignarro, L. J., Cirino, G., Casini, A., & Napoli, C. (1999). Nitric oxide as a signaling molecule. Annual Review of Pharmacology and Toxicology, 39, 1–30.
18. Lawler, A. (2014). Why did the chicken cross the world?: The epic saga of the bird that powers civilization. Atria Books.
19. Li, H., & Poulos, T. L. (2005). Structure-function studies on nitric oxide synthases. Journal of Inorganic Biochemistry, 99(1), 293–305.
20. Lima, D. B., Lima, T. B., Balbuena, T. S., Neves-Ferreira, A. G. C., Barbaro, K. C., & Gozzo, F. C. (2016). Amino acid deprivation disrupts protein synthesis homeostasis and induces survival pathways in Anopheles aquasalis cells. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1863(5), 973–983.
21. Lundberg, J. O., Weitzberg, E., & Gladwin, M. T. (2008). The nitrate-nitrite-nitric oxide pathway. Nature Reviews Drug Discovery, 7(2), 156–167.
22. Morris, S. M. (2004). Enzymes of arginine metabolism. Journal of Nutrition, 134(10), 2743S–2747S.
23. Patel, A. B., Srivastava, S., Phadke, R. S., & Govil, G. (1998). Arginine activates glycolysis of goat epididymal spermatozoa: An NMR study. Biophysical Journal, (3) 75, 1522–1528.
24. Rajfer, J., Aronson, W. J., Bush, P. A., Dorey, F. J., & Ignarro, L. J. (1992). Nitric oxide as a mediator of relaxation. New England Journal of Medicine, 326(2), 90–94.
25. Reilly, C. (2006). Selenium in foods. In C. Reilly (Ed.), Selenium in food and health (pp. 158–172). Springer.
26. Rosselli, M., Keller, P. J., & Dubey, R. K. (1998). Role of nitric oxide in reproduction. Human Reproduction Update, 4(1), 3–24.
27. Silva, L. M. G. S., Murakami, A. E., Fernandez, J. I. M., Dalla Rossa, D., & Urgnani, F. J. (2012). Effects of dietary arginine supplementation on broiler breeder egg production and hatchability. Brazilian Journal of Poultry Science,14(4), 233–304.
28. Soares, R. D. A. T., Fonseca, J. B., Santos, A. S. O., & Mercandante, M. B. (2003). Protein requirement of Japanese quail (Coturnix coturnix japonica) during rearing and laying periods. Brazilian Journal of Poultry Science, 5 153–156.
29. Tamir, H., Ratner, S., & Bengal, E. (2005). Metabolic constraints on arginine biosynthesis in avian species. Journal of Nutrition, 135(5), 1238–1245.
30. Traish, A. M., Toselli, P., Jeong, S. J., & Kim, N. N. (2018). Arginine supplementation in male infertility. International Journal of Impotence Research, 30(1), 1–8.
31. Wu, G., & Morris, S. M. (1998). Arginine metabolism: Nitric oxide and beyond. Annual Review of Nutrition, 18, 61–86.
32. Wu, G., Bazer, F. W., Davis, T. A., Kim, S. W., Li, P., Marc Rhoads, J., Carey Satterfield, M., Smith, S. B., Spencer, T. E., & Yin, Y. (2023). Individual variability in response to arginine. Nutrients, 15(2), 325.
33. Yang, H., Ju, X., Wang, Z., Yang, Z., Lu, J., & Wang, W. (2016). Effects of arginine supplementation on organ development, egg quality, serum biochemical parameters, and immune status of laying hens. Revista Brasileira de Ciência Avícola, 18(1), 181–186.
34. Yang, B., Li, X., Mesalam, N. M., Elsadek, M. F., & Abdel-Moneim, A. M. E. (2024). The impact of dietary supplementation of polysaccharide derived from Polygonatum sibiricum on growth, antioxidant capacity, meat quality, digestive physiology, and gut microbiota in broiler chickens. Poultry Science, 103(6), 103675.
35. Zhou, Q., Li, M., Wang, X., Li, Q., Wang, T., Zhu, Q., Zhou, X., Wang, Y., Gao, T., & Wang, J. (2019). Arginine and nitric oxide synthase in poultry. Poultry Science, 98(11), 5475–5481..
منشور
2026-03-31
How to Cite
الشعبانض. ا., & Hassan, H. (2026). The Effect of Using L-Arginine as a Feed Additive on Testosterone Levels in Ross 308 Broiler Breeder Roosters. Journal of Hama University , 8(4). Retrieved from https://hama-univ.edu.sy/ojs/index.php/huj/article/view/2990
